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Falkenstein Mikrosysteme GmbH plasmid pea29
Plasmid Pea29, supplied by Falkenstein Mikrosysteme GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pea29/plasmid+pea29/10__1007_slash_s42161___024___01596___1-29-17-24
Average 90 stars, based on 1 article reviews
plasmid pea29 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Plasmid Preparation:

Article Title: THIAMIN BIOSYNTHESIS AND ITS INFLUENCE ON EXOPOLYSACCHARIDE PRODUCTION: A NEW COMPONENT OF VIRULENCE IDENTIFIED ON ERWINIA AMYLOVORA PLASMID pEA29
Article Snippet: The ubiquitous plasmid pEA29 of the fire blight pathogen Erwinia amylovora has been well documented as affecting virulence on apple and pear; however, the mechanism of its influence was unknown.. Plasmid-cured strains of E. amylovora exhibit reduced virulence, delayed symptom expression and altered exopolysaccharide (EPS) production on minimal medium with out thiamin (MM-T).. EPS in E. amylovora is composed of two separate fractions, amylovoran (a pathogenicity factor) and levan (a virulence factor), and we have established a link between carriage of pEA29 and elevated expression of the amylovoran biosynthetic operon (ams) during immature pear fruit infection.

Article Title: Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control
Article Snippet: .. Falkenstein, H., Bellemann, P., Walter, S., Zeller, W., and Geider, K. (1988) Identification of Erwinia amylovora, the fireblight pathogen, by colony hybridization with DNA from plasmid pEA29. ..

Article Title: Molecular analyses of Erwinia amylovora strains isolated in Russia, Poland, Slovenia and Austria describing further spread of fire blight in Europe.
Article Snippet: .. Acta Horticult 2006;704:37–42. earch 168 (2013) 447– 454 Falkenstein H, Bellemann P, Walter S, Zeller W, Geider K. Identification of Erwinia amylovora, the fireblight pathogen, by colony hybridization with DNA from plasmid pEA29. .. Appl Environ Microbiol 1988;54:2798–802.

Article Title: Characterisation of naturally occurring Erwinia amylovora strains lacking the common plasmid pEA29 and their detection with real-time PCR
Article Snippet: Erwinia amylovora, the causal agent of fire blight, carries the common plasmid pEA29 of 29 kb.. To screen for occurrence of natural strains without plasmid pEA29, we applied PCR analysis with primers from the plasmid and the chromosomal ams region.. In addition, a described TaqMan probe from pEA29 and newly designed primers from the amsregion were used for identification by real-time PCR.

Article Title: A Novel IncX Plasmid Mediates High-Level Oxytetracycline and Streptomycin Resistance in <i>Erwinia amylovora</i> from Commercial Pear Orchards in California
Article Snippet: Isolates of the fire blight pathogen Erwinia amylovora with high-level resistance to oxytetracycline (minimal inhibitory concentration [MIC] > 100 μg/ml) and to streptomycin (MIC > 100 μg/ml) were recovered from four commercial pear orchards in California between 2018 and 2020.. The two representative oxytetracyclineand streptomycin-resistant (OxyTcRSmR) strains 32-10 and 33-1 were as virulent as the antibiotic susceptible strain 13-1 in causing blossom blight of pear and were recovered more than 50% of the time 7 days after co-inoculation to pear flowers with strain 13-1.. In the field, inoculation of strain 32-10 to pear flowers that were pretreated with oxytetracycline at 200 μg/ml did not reduce disease compared with an untreated control.

Hybridization:

Article Title: Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control
Article Snippet: .. Falkenstein, H., Bellemann, P., Walter, S., Zeller, W., and Geider, K. (1988) Identification of Erwinia amylovora, the fireblight pathogen, by colony hybridization with DNA from plasmid pEA29. ..

Article Title: Molecular analyses of Erwinia amylovora strains isolated in Russia, Poland, Slovenia and Austria describing further spread of fire blight in Europe.
Article Snippet: .. Acta Horticult 2006;704:37–42. earch 168 (2013) 447– 454 Falkenstein H, Bellemann P, Walter S, Zeller W, Geider K. Identification of Erwinia amylovora, the fireblight pathogen, by colony hybridization with DNA from plasmid pEA29. .. Appl Environ Microbiol 1988;54:2798–802.

Clone Assay:

Article Title: Characterisation of naturally occurring Erwinia amylovora strains lacking the common plasmid pEA29 and their detection with real-time PCR
Article Snippet: Erwinia amylovora, the causal agent of fire blight, carries the common plasmid pEA29 of 29 kb.. To screen for occurrence of natural strains without plasmid pEA29, we applied PCR analysis with primers from the plasmid and the chromosomal ams region.. In addition, a described TaqMan probe from pEA29 and newly designed primers from the amsregion were used for identification by real-time PCR.

Selection:

Article Title: Characterisation of naturally occurring Erwinia amylovora strains lacking the common plasmid pEA29 and their detection with real-time PCR
Article Snippet: Erwinia amylovora, the causal agent of fire blight, carries the common plasmid pEA29 of 29 kb.. To screen for occurrence of natural strains without plasmid pEA29, we applied PCR analysis with primers from the plasmid and the chromosomal ams region.. In addition, a described TaqMan probe from pEA29 and newly designed primers from the amsregion were used for identification by real-time PCR.

other:

Article Title: Enhanced colonization and pathogenicity ofErwinia amylovorastrains transformed with the near-ubiquitous pEA29 plasmid on pear and apple
Article Snippet: Almost all wild-type strains of E. amylovora investigated to date do possess a nontransmissable indigenous plasmid known as pEA29 (Falkenstein et al., 1988; Laurent et al., 1989), except in one case in which the 29-kb ubiquitous plasmid was shown to be lacking but replaced by another native plasmid in a Spanish strain (Llop et al., 2006).

Article Title: Genetic characterization of historic Norwegian Erwinia amylovora isolates by SSR-genotyping
Article Snippet: For a long time, all virulent strains of E. amylovora had been assumed to carry a low copy number plasmid of 29 kb (pEa29) (Falkenstein et al. 1988; McGhee and Jones 2000).



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